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JoVE Journal

Neuroscience

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Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction

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08:11 min

August 25th, 2017

August 25th, 2017

11,340 Views

0:05

Title

0:58

Preparation for Injection

2:47

Vector Injection

4:55

Quantification of GFP Expression in the Cerebellum

6:48

Rapid Quantification of the GFP-positive Fluorescent Area in the Cerebellum After Intravenous AAV Administration to the Rat

7:30

Conclusion

Transcript

The overall goal of this adeno-associated virus vector gene transfer is to genetically manipulate the rat's brain and spinal cord on an expansive wide scale basis. This method can help answer key questions in the gene transfer field. It is a very

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Methods for a wide-scale central nervous system gene delivery in the rat are covered. In this example, the purpose is to mimic a disease that affects the entire spinal cord. The widespread transduction can be used to deliver a therapeutic protein to the CNS from a one-time, peripheral administration.

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