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Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

DOI :

10.3791/4031-v

May 24th, 2012

May 24th, 2012

35,375 Views

1Department of Neurology and Hope Center for Neurological Disorders, Washington University School of Medicine

In this protocol we describe production, purification and titration of lentiviral vectors. We provide an example of lentiviral vector-mediated gene delivery in primary cultured neurons and astrocytes. Our methods may also apply to other cell types in vitro and in vivo.

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Lentiviral Vectors

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