Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

17.3K Views

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08:32 min

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July 13th, 2017

DOI :

10.3791/55770-v

July 13th, 2017

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Takahiro Tadokoro¹, Atsushi Miyanohara¹, Michael Navarro¹, Kota Kamizato¹, Stefan Juhas², Jana Juhasova², Silvia Marsala¹, Oleksandr Platoshyn¹, Erik Curtis³, Brandon Gabel³, Joseph Ciacci³, Nada Lukacova⁴, Katarina Bimbova⁴, Martin Marsala¹^,⁴

¹Neuroregeneration Laboratory, Department of Anesthesiology, University of California, San Diego, ²Institute of Animal Physiology and Genetics, Czech Academy of Sciences, ³Department of Neurosurgery, University of California, San Diego, ⁴Institute of Neurobiology, Slovak Academy of Sciences

Summary

Chapters in this video

0:05

Title

1:19

Exposing the Pial Membrane

3:58

Opening the Pial Membrane and Insering the Subpial Needle for AAV9 Delivery

6:11

Results: GFP-expression in Spine Parenchyma and Brain Following Subpial AAV9 Delivery

7:52

Conclusion

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