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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

DOI :

10.3791/56189-v

July 28th, 2017

July 28th, 2017

18,464 Views

1Department of Chemistry, University of Colorado Denver

This article provides a detailed procedure on the solid-phase synthesis, purification, and characterization of dodecamers of RNA modified at the C2'-O-position. UV-vis and circular dichroism photometric analyses are used to quantify and characterize structural aspects, i.e., single-strands or double-strands.

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Keywords Solid phase Synthesis

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