JoVE Logo
Faculty Resource Center

Sign In

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

DOI :

10.3791/57816-v

August 21st, 2018

August 21st, 2018

6,377 Views

1Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder

Proteins that bind specific RNA sequences play critical roles in gene expression. Detailed characterization of these binding sites is crucial for our understanding of gene regulation. Here, a single-step approach for saturation mutagenesis of protein-binding sites in RNA is described. This approach is relevant for all protein-binding sites in RNA.

-- Views

Related Videos

article

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

article

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

article

Detection of Copy Number Alterations Using Single Cell Sequencing

article

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

article

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

article

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

article

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

article

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

article

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

article

An Assay for Quantifying Protein-RNA Binding in Bacteria

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved