August 21st, 2018
•Proteins that bind specific RNA sequences play critical roles in gene expression. Detailed characterization of these binding sites is crucial for our understanding of gene regulation. Here, a single-step approach for saturation mutagenesis of protein-binding sites in RNA is described. This approach is relevant for all protein-binding sites in RNA.
Tags
Related Videos
Novel RNA-Binding Proteins Isolation by the RaPID Methodology
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
Detection of Copy Number Alterations Using Single Cell Sequencing
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved