Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo

10.5K Views

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13:21 min

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September 30th, 2017

DOI :

10.3791/56238-v

September 30th, 2017

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Veronica Akle¹, Nathalie Agudelo-Dueñas*¹^,², Maria A. Molina-Rodriguez*¹, Laurel Brianne Kartchner¹^,³^,⁴^,⁶, Annette Marie Ruth¹^,³^,⁵^,⁶, John M. González³, Manu Forero-Shelton²

¹Laboratory of Neurosciences and Circadian Rhythms, School of Medicine, Universidad de los Andes, ²Biophysics Group, Department of Physics, Universidad de los Andes, ³Laboratory of Basic Medical Sciences, School of Medicine, Universidad de los Andes, ⁴Department of Microbiology and Immunology, University of North Carolina, ⁵Notre Dame Initiative for Global Development, University of Notre Dame, ⁶USAID Research and Innovation Fellowship program

Summary

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Keywords Trypanosoma Cruzi

Light Sheet Fluorescence Microscopy

Transparent Casper Line

Host pathogen Interactions

Chapters in this video

0:00

Title

0:13

Introduction

3:09

Obtaining Embryos for Injection

4:13

Preparing Injection Material

5:38

Obtaining Labeled T. cruzi Parasites for Injection

7:04

Injecting Zebrafish Larvae

8:10

LSFM Mounting and Imaging of Injected Larvae

8:57

LSFM Imaging of Injected Larvae

10:44

Representative Results

11:40

Conclusion

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