Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo
10.6K Views
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13:21 min
•
September 30th, 2017
DOI :
September 30th, 2017
•Veronica Akle1, Nathalie Agudelo-Dueñas*1,2, Maria A. Molina-Rodriguez*1, Laurel Brianne Kartchner1,3,4,6, Annette Marie Ruth1,3,5,6, John M. González3, Manu Forero-Shelton2
1Laboratory of Neurosciences and Circadian Rhythms, School of Medicine, Universidad de los Andes, 2Biophysics Group, Department of Physics, Universidad de los Andes, 3Laboratory of Basic Medical Sciences, School of Medicine, Universidad de los Andes, 4Department of Microbiology and Immunology, University of North Carolina, 5Notre Dame Initiative for Global Development, University of Notre Dame, 6USAID Research and Innovation Fellowship program
Chapters in this video
0:00
Title
0:13
Introduction
3:09
Obtaining Embryos for Injection
4:13
Preparing Injection Material
5:38
Obtaining Labeled T. cruzi Parasites for Injection
7:04
Injecting Zebrafish Larvae
8:10
LSFM Mounting and Imaging of Injected Larvae
8:57
LSFM Imaging of Injected Larvae
10:44
Representative Results
11:40
Conclusion
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