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Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

8.9K Views

10:23 min

December 4th, 2017

DOI :

10.3791/56244-v

December 4th, 2017


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Keywords Endopeptidomic Analysis

Chapters in this video

0:05

Title

1:12

Pre-treatment of Bulk Volume CSF without Quantification

2:24

Pre-treatment of Small Volume CSF Samples with Isobaric Labeling

4:00

MWCO Filtration, Desalting, and Sample Clean-up by Solid Phase Extraction

6:00

Offline High-pH Reverse Phase HPLC Sample Fractionation

7:09

LC-MS Analysis

7:47

Results: Peptide Identification After LC-MS Analysis

9:13

Conclusion

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