JoVE Logo
Faculty Resource Center

Sign In

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

DOI :

10.3791/50589-v

9:20 min

September 2nd, 2013

September 2nd, 2013

27,098 Views

1Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry

Archival formalin fixed and paraffin embedded (FFPE) clinical samples are valuable material for investigation of diseases. Here we demonstrate a sample preparation workflow allowing in-depth proteomic analysis of microdissected FFPE tissue.

Tags

Proteomic Sample Preparation

-- Views

Related Videos

article

Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs

article

Electrophoretic Separation of Proteins

article

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

article

Western Blotting: Sample Preparation to Detection

article

Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles

article

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

article

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

article

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

article

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines

article

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved