Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

DOI :

10.3791/56291-v

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10:55 min

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December 16th, 2017

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8,197 Views

Rosa Gómez-Villafuertes^1,2,3*, Lucía Paniagua-Herranz^1,2,3*, Sergio Gascon^4,5*, David de Agustín-Durán^1,2,3, María de la O Ferreras^1,2,3, Juan Carlos Gil-Redondo^1,2,3, María José Queipo^1,2,3, Aida Menendez-Mendez^1,2,3, Ráquel Pérez-Sen^1,2,3, Esmerilda G. Delicado^1,2,3, Javier Gualix^1,2,3, Marcos R. Costa⁶, Timm Schroeder⁷, María Teresa Miras-Portugal^1,2,3, Felipe Ortega^1,2,3

¹Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University, ²University Institute for Neurochemistry Research (IUIN), ³Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), ⁴Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilians University Munich, ⁵Toxicology and Pharmacology Department, Faculty of Veterinary medicine, Complutense University, ⁶Brain Institute, Federal University of Rio Grande do Norte, ⁷Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich

Chapters

A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. This method represents a powerful tool to identify biological events in a selected population during live imaging experiments.

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