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Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

DOI :

10.3791/56063-v

August 9th, 2017

August 9th, 2017

7,821 Views

1Brain Institute, Federal University of Rio Grande do Norte, 2Department of Biosystems Science and Engineering, ETH Zurich, 3The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University

Live imaging is a powerful tool to study cellular behaviors in real time. Here, we describe a protocol for time-lapse video-microscopy of primary cerebral cortex cells that allows a detailed examination of the phases enacted during the lineage progression from primary neural stem cells to differentiated neurons and glia.

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Keywords Live Imaging

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