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Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

DOI :

10.3791/56313-v

November 13th, 2017

November 13th, 2017

17,546 Views

1The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University

Assay for Transposase-Accessible Chromatin coupled with high-throughput sequencing (ATAC-seq) is a genome-wide method to uncover accessible chromatin. This is a step-by-step ATAC-seq protocol, from molecular to the final computational analysis, optimized for human lymphocytes (Th1/Th2). This protocol can be adopted by researchers without prior experience in next-generation sequencing methods.

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