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Methods for Imaging Intracellular pH of the Follicle Stem Cell Lineage in Live Drosophila Ovarian Tissue

DOI :

10.3791/56316-v

September 26th, 2017

September 26th, 2017

6,671 Views

1Departments of Anatomy and OB/GYN-RS, University of California, San Francisco

We provide a protocol for imaging intracellular pH of an epithelial stem cell lineage in live Drosophila ovarian tissue. We describe methods to generate transgenic flies expressing a pH biosensor, mCherry::pHluorin, image the biosensor using quantitative fluorescence imaging, generate standard curves, and convert fluorescence intensity values to pH values.

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Intracellular PH

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