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Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

DOI :

10.3791/56333-v

November 23rd, 2017

November 23rd, 2017

12,037 Views

1Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics, University of Southern California, 2The Saban Research Institute of Children's Hospital Los Angeles, 3Department of Biochemistry and Molecular Medicine, University of Southern California

This protocol serves as a scheme for setting up a functional Tet-ON system in cancer cell lines and its subsequent use, in particular for studying the role of tumor cell-derived proteins in recruitment of monocytes/macrophages to the tumor microenvironment.

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Conditional Knockdown

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