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Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration

DOI :

10.3791/57525-v

April 27th, 2018

April 27th, 2018

8,426 Views

1Department of Clinical Medicine, Aarhus University, 2GDD, Cancer Cell Biology Program, National Cancer Research Center (CNIO)

This protocol describes a newly established method for virus delivery to the murine prostate. Using either CRISPR/Cas9 technology, gene overexpression, or Cre recombinase delivery, the technique allows orthotopic alteration of gene expression and implements a novel mouse model for prostate cancer.

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Virus Delivery

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