January 11th, 2019
•Establishing a stable cell line overexpressing a gene of interest to study gene function can be done by stable transfection-picking single clones after transfecting them via retroviral infection. Here we show that HT29-DR3 cell lines generated in this way elucidate the mechanisms by which death receptor 3 (DR3) contributes to antimitotics-induced apoptosis.
Related Videos
Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis
Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel
Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment
Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy
Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
Analysis of Side Population in Solid Tumor Cell Lines
Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs
Transmitochondrial Cybrid Generation Using Cancer Cell Lines
Optimization of Performance Parameters of the TAGGG Telomere Length Assay
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved