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One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy

DOI :

10.3791/56338-v

6:47 min

October 23rd, 2017

October 23rd, 2017

10,762 Views

1Department of Radiation Oncology, Gunma University Graduate School of Medicine, 2Advanced Scientific Research Leaders Development Unit, Gunma University Graduate School of Medicine

Research on ionizing radiation (IR)-induced clonogenic cell death is important for understanding the effects of IR on malignant tumors and normal tissues. Here, we describe a one-step assay for assessing the major modes of IR-induced clonogenic cell death based on morphology of 4',6-diamidino-2-phenylindole dihydrochloride (DAPI)-stained nuclei visualized by fluorescence microscopy.

Tags

Fluorescence Microscopy

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