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Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

DOI :

10.3791/56343-v

November 1st, 2017

November 1st, 2017

9,987 Views

1Department of Medical Engineering, California Institute of Technology, 2Department of Earth Sciences, University of Southern California, 3Jet Propulsion Laboratory, California Institute of Technology

Digital holographic microscopy (DHM) is a volumetric technique that allows imaging samples 50-100X thicker than brightfield microscopy at comparable resolution, with focusing performed post-processing. Here DHM is used for identifying, counting, and tracking microorganisms at very low densities and compared with optical density measurements, plate count, and direct count.

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Microorganisms

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