Preparation of Highly Dilute Samples for DHM and Recording DHM Videos
4:06
Calculations of Cell Density and Limits of Detection
5:22
Results: Growth Curves and Representative DHM Data
6:14
Conclusion
필기록
The overall goal of this experiment is to demonstrate the detection of microorganisms at very low levels using digital holographic microscopy. This technique is more sensitive than traditional light microscopy and provides real-time information ab
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Digital holographic microscopy (DHM) is a volumetric technique that allows imaging samples 50-100X thicker than brightfield microscopy at comparable resolution, with focusing performed post-processing. Here DHM is used for identifying, counting, and tracking microorganisms at very low densities and compared with optical density measurements, plate count, and direct count.