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Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System

9.3K Views

10:11 min

January 31st, 2018

DOI :

10.3791/56421-v

January 31st, 2018


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Mycobacterium Bovis BCG

Chapters in this video

0:05

Title

1:00

Monomeric Avidin Fusion Protein Expression, Purification, and Refolding

3:53

Biotinylation of BCG Cell Surface

5:47

Binding of Monomeric Adivin-fusion Protein to Biotinylated BCG Surface

7:06

Results: Biotinylation with Avidin-OVA

9:13

Conclusion

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