JoVE Logo
Faculty Resource Center

Sign In

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

DOI :

10.3791/56430-v

August 3rd, 2017

August 3rd, 2017

6,342 Views

1Sackler Faculty of Medicine, Tel Aviv University, 2Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 3Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University, 4Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University

A simple protocol for overexpression and purification of codon-optimized, human cis-prenyltransferase, under non-denaturing conditions, from Escherichia coli, is described, along with an enzymatic activity assay. This protocol can be generalized for production of other cis- prenyltransferase proteins in quantity and quality suitable for mechanistic studies.

Tags

Overexpression

-- Views

Related Videos

article

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

article

Following Cell-fate in E. coli After Infection by Phage Lambda

article

Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes

article

piggyBac Transposon System Modification of Primary Human T Cells

article

Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat

article

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode

article

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

article

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System

article

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

article

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved