Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli
6.5K Views
•
07:35 min
•
August 3rd, 2017
DOI :
August 3rd, 2017
•Ilan Edri*1, Michal Goldenberg*1, Michal Lisnyansky2, Roi Strulovich2, Hadas Newman1,3, Anat Loewenstein1,3, Daniel Khananshvili2, Moshe Giladi2,4, Yoni Haitin2
1Sackler Faculty of Medicine, Tel Aviv University, 2Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 3Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University, 4Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University
Chapters in this video
0:05
Title
0:56
Purification of Human DHDDS
3:52
Analytical Size-exclusion Chromatography (SEC)
4:40
Enzyme Kinetics – Time-dependent Activity
6:15
Results: Purification and Analysis of Human DHDDS
7:00
Conclusion
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