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Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

6.5K Views

11:25 min

October 4th, 2017

DOI :

10.3791/56302-v

October 4th, 2017


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Cysteine Thiols

Chapters in this video

0:05

Title

1:09

Polymerase Chain Reaction (PCR)-mediated Site-directed Mutagenesis

3:44

Uniform 15N-labeled Protein Expression in BL21 ΔE3 E. coli

5:24

Purification of Recombinant Protein from E. coli

7:50

Chemical Attachment of Glucose-5-MTS to Protein by Dialysis

8:48

Results: Site-selective Cys Mutation Followed by In Vitro Glycosylation in STIM1 EF-SAM Protein

10:11

Conclusion

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