Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
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11:25 min
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October 4th, 2017
DOI :
October 4th, 2017
•Chapters in this video
0:05
Title
1:09
Polymerase Chain Reaction (PCR)-mediated Site-directed Mutagenesis
3:44
Uniform 15N-labeled Protein Expression in BL21 ΔE3 E. coli
5:24
Purification of Recombinant Protein from E. coli
7:50
Chemical Attachment of Glucose-5-MTS to Protein by Dialysis
8:48
Results: Site-selective Cys Mutation Followed by In Vitro Glycosylation in STIM1 EF-SAM Protein
10:11
Conclusion
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