tel aviv university

9 ARTICLES PUBLISHED IN JoVE

Behavior

Applying Incongruent Visual-Tactile Stimuli during Object Transfer with Vibro-Tactile Feedback

Jason Friedman¹, Eitan Raveh², Tehilla Weiss², Sophi Itkin², Dafna Niv², Mor Hani², Sigal Portnoy²

¹Department of Physical Therapy, Sackler Faculty of Medicine, Tel Aviv University, ²Department of Occupational Therapy, Sackler Faculty of Medicine, Tel Aviv University

We present a protocol to apply incongruent visual-tactile stimuli during an object transfer task. Specifically, during block transfers, performed while the hand is hidden, a virtual presentation of the block shows random occurrences of false block drops. The protocol also describes adding vibrotactile feedback while performing the motor task.

Neuroscience

Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

Topaz Altman^*1, Roy Maimon^*1, Ariel Ionescu^*1, Tal Gradus Pery¹, Eran Perlson^1,2

¹Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, ²Sagol School of Neuroscience, Tel Aviv University

Axonal transport is a crucial mechanism for motor neuron health. In this protocol we provide a detailed method for tracking the axonal transport of acidic compartments and mitochondria in motor neuron axons using microfluidic chambers.

Engineering

Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments

Yonatan Gershuni^1,2, Michal Elkind^1,2, Dolev Roitman^1,2, Itamar Cohen^1,2, Aviad Tsabary^1,2, Deep Sarkar^1,2, Ishay Pomerantz^1,2

¹The School of Physics and Astronomy, Tel Aviv University, ²Tel Aviv University Center for Light-Matter Interaction

A protocol is presented for automated irradiation of thin gold foils with high intensity laser pulses. The protocol includes a step-by-step description of the micromachining target fabrication process and a detailed guide for how targets are brought to the laser's focus at a rate of 0.2 Hz.

Genetics

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Sampath Kumar Loganathan¹, Ahmad Malik^1,2, Ellen Langille^1,2, Chen Luxenburg³, Daniel Schramek^1,2

¹Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, ²Department of Molecular Genetics, University of Toronto, ³Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent mice.

JoVE Core

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Lael Werner^1,2, Chen Dor^3,4, Naomi Salamon¹, Meital Nagar^3,4, Dror S. Shouval^1,2

¹Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, ²Sackler Faculty of Medicine, Tel Aviv University, ³Cancer Research Center, Sheba Medical Center, ⁴Molecular Hematology Laboratory, Sheba Medical Center

The current protocol describes a method for DNA isolation from blood samples and intestinal biopsies, generation of TCRβ and IGH PCR libraries for next-generation sequencing, performance of a NGS run and basic data analysis.

Cancer Research

Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs

Neta Moskovits^1,2, Ella Itzhaki^1,2, Nataly Tarasenko^1,2, Eva Chausky^1,2, Avital Bareket-Samish³, Aleksandr Kaufman^1,2, Raisa Meerson^1,2, Salomon M. Stemmer^1,2,4

¹Felsenstein Medical Research Center, ²Davidoff Center, Rabin Medical Center, ³BioInsight Ltd., ⁴Sackler Faculty of Medicine, Tel Aviv University

The present protocol describes generating 3D tumor culture models from primary cancer cells and evaluating their sensitivity to drugs using cell-viability assays and microscopic examinations.

Endless Worms Most Beautiful: Current Methods For Using Nematodes To Study Evolutionary Developmental Biology

Chee Kiang Ewe¹, Pradeep M. Joshi², Joel H. Rothman²

¹Department of Neurobiology, Tel Aviv University, ²Department of Molecular, Cellular, Developmental Biology, University of California Santa Barbara

Endless Worms Most Beautiful: Current Methods For Using Nematodes To Study Evolutionary Developmental Biology

JoVE Journal

Visualizing the DNA Damage Response in Purkinje Cells Using Cerebellar Organotypic Cultures

Sharone Naor¹, Yael Ziv¹, Yosef Shiloh¹

¹The David and Inez Myers Laboratory for Cancer Genetics, Department of Human Molecular Genetics and Biochemistry, Faculty of Health and Medical Sciences, School of Medicine, Tel Aviv University

Cerebellar Purkinje cells (PCs) are particularly sensitive to deficiencies in the DNA damage response. A protocol is presented for the visual evaluation of the dynamics of PC response to DNA damage, which involves staining protein-bound poly(ADP-ribose) chains within cerebellar organotypic cultures.

Bioengineering

Capturing Dynamic Finger Gesturing with High-resolution Surface Electromyography and Computer Vision

Nitzan Luxembourg¹, Dvir Ben-Dov¹, Rufael Fekadu Marew², Dvir Teitelbaum¹, Ieva Vebraite Adereth¹, Yael Hanein^1,3

¹School of Electrical Engineering, Tel Aviv University, ²Department of Machine Learning, Mohamed bin Zayed University of Artificial Intelligence (MBZUAI), ³X-trodes Ltd.

The paper presents a comprehensive protocol for simultaneously recording hand-electromyography (EMG) and visual finger tracking during natural finger gesturing. The visual data is designed to serve as the ground truth for the development of accurate EMG-based computational models for finger gesture recognition.