Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

10.6K Views

•

09:52 min

•

December 28th, 2017

DOI :

10.3791/56436-v

December 28th, 2017

•

Benjamin B. Fixman*¹, Isaac W. Babcock*¹, Laurie S. Minamide*¹, Alisa E. Shaw¹, Marina I. Oliveira da Silva¹^,², Avery M. Runyan¹, Michael T. Maloney¹^,³, Jeffrey J. Field¹, James R. Bamburg¹

¹Department of Biochemistry and Molecular Biology and Molecular, Cellular and Integrated Neuroscience Program, Colorado State University, ²IBMC-Instituto de Biologia Molecular e Celular, i3S-Instituto de Investigaçãoe Inovação em Saúde, ICBAS, Universidade do Porto, ³Denali Therapeutics

Summary

Explore More Videos

Brain Slice Culture

Long term Culture

Repetitive Imaging

Fluorescence Imaging

Enclosed System

Roller Tube Method

Photo Etched Coverslips

Neurological Disorders

Synapse Alteration

Chapters in this video

0:05

Title

1:28

Preparation of Roller Tubes and Coverslips

2:51

Plating Slices

5:44

Slice Imaging

7:28

Results: Repetitive Intermittent Imaging of Brain Slices During Long-term Culture

8:49

Conclusion

Related Videos

article

Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring

10.3K Views

article

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

16.3K Views

article

Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices

17.9K Views

article

Modification of a Colliculo-thalamocortical Mouse Brain Slice, Incorporating 3-D printing of Chamber Components and Multi-scale Optical Imaging

8.3K Views

article

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices

9.8K Views

article

Comprehensive Autopsy Program for Individuals with Multiple Sclerosis

11.4K Views

article

Horizontal Hippocampal Slices of the Mouse Brain

17.8K Views

article

Time-Lapse Imaging of Migrating Neurons and Glial Progenitors in Embryonic Mouse Brain Slices

795 Views

article

A Modified Roller Tube Method for the Long-Term Culture of Rodent Brain Slices

21 Views

article

Two-Photon Microscopy for Studying Microglial Process Attraction Toward a Compound in a Mouse Brain Slice

18 Views

Copyright © 2025 MyJoVE Corporation. All rights reserved