Results: Immunostained Exosome Sections Provide Insight to Their Protein Makeup
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Conclusion
Transcript
The overall goal of this procedure is to observe the morphology of purified extracellular vesicles and to perform specific protein localization within extracellular vesicles using electron microscopy. This method can help answer key questions in t
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This protocol describes the various techniques necessary for transmission electron microscopy including negative staining, ultrathin sectioning for detailed structure, and immuno-gold labelling to determine the positions of specific proteins in exosomes.