Establishing 3D Co-cultures with Indirect Interaction
2:13
3D Co-cultures with Direct Interaction
6:14
Characterization of the Effects of Supernatants from Primary BrC Cells on Acini Formation and Acini Structure
8:28
Results: Communication between Monocytes and Primary BrC Cells in an ECME-3D System
10:01
Conclusion
Transcript
The overall goal of this protocol is to study the direct and indirect communication between primary breast cancer cells and monocytes in a three-dimensional co-culture system. This method can help answer key questions in inflammatory microenvironm
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Here, we describe a three-dimensional culture method to analyze the morphology of primary breast cancer cells, as well as to study their direct/indirect interactions with monocytes and the outcomes such as collagen degradation, immune cell recruitment, cell invasion, and promotion of cancer-related inflammation.