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Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model

DOI :

10.3791/51341-v

June 11th, 2014

June 11th, 2014

15,513 Views

1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, 2Department of Oncology, Schulich School of Medicine and Dentistry, University of Western Ontario, 3Lawson Health Research Institute

This article provides detailed methodologies for the use of three-dimensional (3D) assays to quantify breast cancer cell invasion. Specifically, we discuss the procedures required to set up such assays, quantification, and data analysis, as well as methods to examine the loss of membrane integrity that occurs when cells invade.

Tags

Breast Cancer

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