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Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System

DOI :

10.3791/56589-v

January 8th, 2018

January 8th, 2018

10,791 Views

1Unidad de Investigación en Virología y Cáncer, Hospital Infantil de México Federico Gómez, 2Programa de Doctorado en Ciencias Biomédicas, Facultad de Medicina, Universidad Nacional Autónoma de México

Here, we describe a three-dimensional culture method to analyze the morphology of primary breast cancer cells, as well as to study their direct/indirect interactions with monocytes and the outcomes such as collagen degradation, immune cell recruitment, cell invasion, and promotion of cancer-related inflammation.

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Keywords 3D Co culture

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