Results: Nuclease Assay of Heparin Sepharose Fractions
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Conclusion
Transcript
The overall goal of this protocol is to identify and remove contaminating nuclease activity during protein purification. This method can help answer key questions in the retrovirology field, such as biochemistry, structure, and dynamics of integra
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Recombinant prototype foamy virus integrase protein is often contaminated with a bacterial nuclease during purification. This method identifies nuclease contamination and removes it from the final preparation of the enzyme.