Rescue of Recombinant Newcastle Disease Virus from cDNA

19.2K Views

10:55 min

October 11th, 2013

DOI :

10.3791/50830-v

October 11th, 2013


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Newcastle Disease Virus

Chapters in this video

0:05

Title

1:37

Infection of Mammalian Cells with the Recombinant Modified Vaccinia Ankara Virus Expressing the Bacteriophage T7 RNA Polymerase (MVA-T7)

2:40

Transfection of Mammalian Cells

3:48

Co-culture of Mammalian Cells with Avian Cells

5:22

Infection of Chicken Embryonated Eggs

6:31

Harvest of Allentoic Fluid from Infected Chicken Embryonated Eggs

7:52

Hemagglutination (HA) Assay

9:03

Results: Transfection and MVA-T7 Infection Efficiencies and Hemagglutination Assay

10:33

Conclusion

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