Results: Confocal Microscopy and 2D and 3D Mixed Cultures
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Conclusion
Transcript
The overall goal of this 3D cell culture protocol is to model the glial scar event of the central nervous system using key indicator cells:microglia, astrocytes and oligodendrocytes.This method can help answer key questions in the neuro-inflammati
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Herein, we present a protocol for the 3D culture of rat brain-derived glia cells, including astrocytes, microglia, and oligodendrocytes. We demonstrate primary cell culture, methacrylated hyaluronic acid (HAMA) hydrogel synthesis, HAMAphoto-polymerization and cell encapsulation, and sample processing for confocal and scanning electron microscopic imaging.