JoVE Logo
Faculty Resource Center

Sign In

Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder (ALLIGATOR)

DOI :

10.3791/56623-v

10:33 min

December 13th, 2017

December 13th, 2017

7,496 Views

1MRC Laboratory of Molecular Biology

Genetically encoded luciferase is a popular non-invasive reporter of gene expression. Use of an automated longitudinal luciferase imaging gas- and temperature-optimized recorder (ALLIGATOR) enables longitudinal recording from bioluminescent cells under a wide range of conditions. Here we show how ALLIGATOR may be used in the context of circadian rhythms research.

Tags

Bioluminescent Reporters

-- Views

Related Videos

article

In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum

article

Development of automated imaging and analysis for zebrafish chemical screens.

article

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

article

LeafJ: An ImageJ Plugin for Semi-automated Leaf Shape Measurement

article

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

article

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

article

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

article

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

article

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

article

Measuring the Confluence of iPSCs Using an Automated Imaging System

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved