Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
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10:38 min
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September 27th, 2012
DOI :
September 27th, 2012
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Chapters in this video
0:05
Title
2:01
Construction of Lentiviral Luciferase Reporters
2:28
Production of Lentiviral Particles: Seed Cells (Day 1)
3:05
Transient Transfection: CaPO4/DNA Precipitation (Day 2)
4:23
Harvest Viral Particles (Days 3-4)
5:02
Infection of 3T3 Cells (Day 3 Onward)
6:12
Synchronization and Change to Recording Medium
7:02
Bioluminescence Recording of Reporter Cells
7:26
Data Analysis and Presentation
8:12
Results: Phase-specific Circadian Reporters
9:47
Conclusion
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