JoVE Logo
Faculty Resource Center

Sign In

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

DOI :

10.3791/56631-v

January 26th, 2018

January 26th, 2018

7,302 Views

1Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine, University of Freiburg, 2Faculty of Biology, University of Freiburg

We present an effective and reproducible method to isolate and culture neural progenitor cells from embryonic and postnatal brain tissue for chromatin immunoprecipitation (ChIP) of histone 3 lysine 79 dimethylation (H3K79me2) - a histone mark located within the globular domain of histone 3.

Tags

Keywords Neural Progenitors

-- Views

Related Videos

article

Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells

article

Physiological, Morphological and Neurochemical Characterization of Neurons Modulated by Movement

article

Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury

article

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

article

Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis

article

Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol

article

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

article

An Optimized Procedure for Fluorescence-activated Cell Sorting (FACS) Isolation of Autonomic Neural Progenitors from Visceral Organs of Fetal Mice

article

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury

article

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved