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Neuronal Nuclei Isolation from Human Postmortem Brain Tissue Video (Video) | JoVE

DOI :

10.3791/914-v

October 1st, 2008

October 1st, 2008

21,684 Views

1Psychiatry, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School

The cellular heterogeneity of brain tissue poses a significant limitation for the study of epigenetic markings in chromatin because most assays lack single cell resolution. Neurons typically are intermingled with glia and other non-neuronal cells. We provide a protocol to extract and collect neuronal nuclei from human brain.

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