Native Chromatin Immunoprecipitation Using Murine Brain Tumor Neurospheres
7.6K Views
•
09:31 min
•
January 29th, 2018
DOI :
January 29th, 2018
•Flor M. Mendez1, Felipe J. Núñez1,2, Rocío I. Zorrilla-Veloz3,4, Pedro R. Lowenstein1,2, Maria G. Castro1,2
1Department of Cell and Developmental Biology, University of Michigan Medical School, 2Department of Neurosurgery, University of Michigan Medical School, 3Cancer Research Summer Internship Program (CARSIP), Cancer Biology Program, University of Michigan Medical School, 4Department of Biology, University of Puerto Rico-Río Piedras Campus
Chapters in this video
0:05
Title
0:40
Generation of Brain Tumor Neurospheres and Culturing Conditions
2:23
Chromatin Preparation
4:52
Immunoprecipitation (IP)
8:21
Results: Representative ChIP qPCR Data as Percent Input
9:05
Conclusion
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