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JoVE Journal

Medicine

This content is Open Access.

Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature

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10:07 min

December 26th, 2017

December 26th, 2017

12,659 Views

0:05

Title

0:54

Fixation of Zebrafish Tissue

1:41

Preparation of the Zebrafish Retinal Vasculature

6:39

Mounting and Visualization of the Retinal Vasculature

7:51

Results: Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature

9:30

Conclusion

Transcript

Here, we discuss a method protocol which will allow an easy analysis of the adult tg(fli:EGFP) zebrafish retinal vasculature as a fast read-out in settings of long-term vascular pathologies linked to neoangiogenesis and structural changes.

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