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Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

DOI :

10.3791/55335-v

February 24th, 2017

February 24th, 2017

8,295 Views

1Department of Biological Sciences, University of Notre Dame, 2Department of Neurosurgery, Mayo Clinic

Zebrafish retinal regeneration has mostly been studied using fixed retinas. However, dynamic processes such as interkinetic nuclear migration occur during the regenerative response and require live-cell imaging to investigate the underlying mechanisms. Here, we describe culture and imaging conditions to monitor Interkinetic Nuclear Migration (INM) in real-time using multiphoton microscopy.

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