JoVE Logo
Faculty Resource Center

Sign In

Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

DOI :

10.3791/55335-v

February 24th, 2017

February 24th, 2017

8,329 Views

1Department of Biological Sciences, University of Notre Dame, 2Department of Neurosurgery, Mayo Clinic

Zebrafish retinal regeneration has mostly been studied using fixed retinas. However, dynamic processes such as interkinetic nuclear migration occur during the regenerative response and require live-cell imaging to investigate the underlying mechanisms. Here, we describe culture and imaging conditions to monitor Interkinetic Nuclear Migration (INM) in real-time using multiphoton microscopy.

Tags

Adult Transgenic Zebrafish

-- Views

Related Videos

article

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

article

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation

article

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

article

Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System

article

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

article

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

article

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences

article

Live Imaging of Mouse Secondary Palate Fusion

article

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

article

Live Cell Imaging of Chromosome Segregation During Mitosis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved