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Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model

DOI :

10.3791/50489-v

June 13th, 2013

June 13th, 2013

17,963 Views

1Department of Ophthalmology, Northwestern University Feinberg School of Medicine

Trypsin digest is one of the most commonly used methods to analyze retinal vasculature. This manuscript describes the method in detail, including key alterations to optimize the technique and remove the non-vascular tissue while preserving the overall architecture of the vessels.

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Trypsin Digest Protocol

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