JoVE Logo

Sign In

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

13.3K Views

12:34 min

April 2nd, 2018

DOI :

10.3791/56739-v

April 2nd, 2018


Explore More Videos

Two dimensional Gel Electrophoresis

Chapters in this video

0:04

Title

1:46

Two-dimensional Gel Electrophoresis: IEF (First Dimension)

3:12

Two-dimensional Gel Electrophoresis: SDS-PAGE (Second Dimension)

7:19

Staining of 2DE-separated Proteins

8:52

Results: Separation and Identification of NFPA Tissue Proteome

10:33

Conclusion

Related Videos

article

09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

13.7K Views

article

08:11

Quantitative Analysis of Chromatin Proteomes in Disease

13.0K Views

article

10:51

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

16.0K Views

article

10:18

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

13.6K Views

article

08:08

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

16.0K Views

article

08:59

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

205 Views

article

09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

5.5K Views

article

11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

4.4K Views

article

07:29

Separating Protein with SDS-PAGE

478.4K Views

article

07:31

Two-Dimensional Gel Electrophoresis

50.3K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved