Two-dimensional Gel Electrophoresis: IEF (First Dimension)
3:12
Two-dimensional Gel Electrophoresis: SDS-PAGE (Second Dimension)
7:19
Staining of 2DE-separated Proteins
8:52
Results: Separation and Identification of NFPA Tissue Proteome
10:33
Conclusion
필기록
The overall goal of this two-dimensional gel electrophoresis coupled with mass spectrometry procedure is to separate and identify human pituitary adenoma tissue proteome. This method can help answer key questions in the proteomes field. Such as hi
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
Here, we present a two-dimensional gel electrophoresis (2DE) coupled with mass spectrometry (MS) to separate and identify human pituitary adenoma tissue proteome, which presents a good and reproducible 2DE pattern. Many proteins are observed in each 2DE spot when analyzing complex cancer proteome with the use of high-sensitivity MS.