A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins
13.6K Views
•
14:49 min
•
June 12th, 2018
DOI :
June 12th, 2018
•Chapters in this video
0:04
Title
0:46
Production of AAV in HEK 293 Cells by Plasmid DNA Transfection
2:50
Recovery of Viral Particles, and Treatment with Serratia marcescens Endonuclease and Proteinase K
4:20
Phenol-choloroform Extraction, Ethanol Precipitation, and Preparation of Plasmid DNA Standards
5:55
Dot Blot Assay
8:46
Hybridization and Washing
10:50
Results: AAV Titration and Functional Assessment of AAP Proteins Using a Quantitative Dot Blot Assay
12:58
Conclusion
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