JoVE Logo
Faculty Resource Center

Sign In

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

DOI :

10.3791/63038-v

December 25th, 2021

December 25th, 2021

4,049 Views

1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Nanotechnology Research and Application Center (SUNUM), Sabanci University

This protocol presents how to quantify AAV transduction efficiency in mouse retina using digital droplet PCR (dd-PCR) together with small scale AAV production, intravitreal injection, retinal imaging, and retinal genomic DNA isolation.

-- Views

Related Videos

article

In vivo Electroporation of Developing Mouse Retina

article

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

article

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

article

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

article

Ocular Kinematics Measured by In Vitro Stimulation of the Cranial Nerves in the Turtle

article

A Volumetric Method for Quantification of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage

article

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

article

Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

article

In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility

article

Quantification of Immunostained Caspase-9 in Retinal Tissue

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved