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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

DOI :

10.3791/59086-v

April 11th, 2019

April 11th, 2019

19,717 Views

1Genome Institute of Singapore, Agency for Science Technology and Research, 2School of Chemical and Biomedical Engineering, Nanyang Technological University

CRISPR-Cas is a powerful technology to engineer the complex genomes of plants and animals. Here, we detail a protocol to efficiently edit the human genome using different Cas endonucleases. We highlight important considerations and design parameters to optimize editing efficiency.

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