April 11th, 2019
•CRISPR-Cas is a powerful technology to engineer the complex genomes of plants and animals. Here, we detail a protocol to efficiently edit the human genome using different Cas endonucleases. We highlight important considerations and design parameters to optimize editing efficiency.
Related Videos
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved