Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay

8.2K Views

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10:41 min

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March 7th, 2018

DOI :

10.3791/56846-v

March 7th, 2018

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Bo Han¹, Man Zhang², Peng Sun¹, Shangwei Hou³

¹Department of General Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, ²Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine, Shanghai Jiao Tong University, ³Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine

Summary

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Keywords Bio layer Interferometry

BLI Assay

Ion Channel

Protein small Molecule Interaction

Affinity Chromatography

Chapters in this video

0:04

Title

1:01

Affinity Purification of FLAG-tagged hEAG1 Channel Protein from HEK-293 Cells

4:18

BCA Assay, Western Blotting, and Labeling the Purified Protein with Biotin for the BLI Assay

6:10

Bio-layer Interferometry Assay

8:06

Results: Interaction Kinetics of hEAG1 with PIP2 Using the Bio-layer Interferometry Assay

9:35

Conclusion

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