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Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

DOI :

10.3791/58228-v

January 7th, 2019

January 7th, 2019

6,154 Views

1Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, 2Department of Emergency Medical Services, Eulji University, 3Department of Mechanical Engineering, Sungkyunkwan University

Mechanosensitive ion channels are often studied in terms of fluid flow/shear force sensitivity with patch-clamp recording. However, depending on the experimental protocol, the outcome on fluid flow-regulations of ion channels can be erroneous. Here, we provide methods for preventing and correcting such errors with a theoretical basis.

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