Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH
6.9K Views
•
10:56 min
•
December 21st, 2017
DOI :
December 21st, 2017
•Chapters in this video
0:05
Title
1:34
Immuno-DNA FISH (Fluorescent In Situ Hybridization) on Wire
2:35
Fixation, Permeabilization, and Immunofluorescence
4:25
3D DNA FISH Day 2
6:08
3D DNA FISH Day 3
7:51
3D Microscope Observation and Analysis
9:01
Results: No Interference Bewteen Immunofluorescence and DNA FISH Signals
9:38
Conclusion
Related Videos
Capture and Release of Viable Circulating Tumor Cells from Blood
8.4K Views
The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics
11.0K Views
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer
9.9K Views
Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
8.8K Views
The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
9.2K Views
Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
10.8K Views
Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
8.5K Views
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
8.5K Views
Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
10.5K Views
Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
10.3K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved