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Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture

DOI :

10.3791/61392-v

June 23rd, 2020

June 23rd, 2020

10,773 Views

1Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University

The presented approach simultaneously evaluates cancer cell invasion in 3D spheroid assays and T-cell cytotoxicity. Spheroids are generated in a scaffold-free agarose multi-microwell cast. Co-culture and embedding in type I collagen matrix are performed within the same device which allows to monitor cancer cell invasion and T-cell mediated cytotoxicity.

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Keywords 3D Culture

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