Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro

9.5K Views

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11:03 min

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January 31st, 2018

DOI :

10.3791/57044-v

January 31st, 2018

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Kaela M. Varberg¹^,², Seth Winfree³^,⁴, Kenneth W. Dunn³^,⁴, Laura S. Haneline¹^,²^,⁵^,⁶^,⁷

¹Department of Cellular and Integrative Physiology, Indiana University School of Medicine, ²Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, ³Indiana Center for Biological Microscopy, Indiana University School of Medicine, ⁴Department of Medicine, Indiana University School of Medicine, ⁵Department of Pediatrics, Indiana University School of Medicine, ⁶Department of Microbiology and Immunology, Indiana University School of Medicine, ⁷Indiana University Simon Cancer Center, Indiana University School of Medicine

Summary

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Keywords Vasculogenesis

Network Structural Patterns

Cell Population

Pathologic State

Microscope Stage Top Incubator

Basement Membrane Matrix

Matrigel

Pipetting

Chapters in this video

0:05

Title

0:59

Microscope Preparation and Endothelial Colony Forming Cell (ECFC) Matrix Plating

4:46

Image Acquisition and Processing and Kinetic Analysis of Vasculogenesis (KAV)

8:12

Results: Representative Kinetic Analysis of Vasculogenesis

10:22

Conclusion

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