JoVE Logo
Faculty Resource Center

Sign In

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

DOI :

10.3791/57109-v

June 15th, 2018

June 15th, 2018

7,234 Views

1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3RCSI Molecular Medicine, Royal College of Surgeons in Ireland, 4Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences, University of New South Wales, 6St Vincent's Hospital Clinical School, University of New South Wales, 7School of Medicine and Medical Science, University College Dublin

This manuscript describes the protocol for Bimolecular Complementation Affinity Purification (BiCAP). This novel method facilitates the specific isolation and downstream proteomic characterization of any two interacting proteins, while excluding un-complexed individual proteins as well as complexes formed with competing binding partners.

Tags

Multi protein Signaling Complexes

-- Views

Related Videos

article

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

article

Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method

article

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

article

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

article

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells

article

Purification of Pathogen Vacuoles from Legionella-infected Phagocytes

article

Real-time Live Imaging of T-cell Signaling Complex Formation

article

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

article

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

article

Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved