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Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

DOI :

10.3791/57225-v

April 27th, 2018

April 27th, 2018

13,389 Views

1Department of Pediatrics, Emory University School of Medicine, 2Emory College of Arts and Sciences, Emory University

Here, we present two protocols for the measurement of mitochondrial Ca2+ influx in isolated mitochondria and cultured cells. For isolated mitochondria, we detail a plate reader-based Ca2+ import assay using the Ca2+ sensitive dye calcium green-5N. For cultured cells, we describe a confocal microscopy method using the Ca2+ dye Rhod-2/AM.

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Mitochondrial Calcium Influx

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